New utility of herbal samples for retrospective biomonitoring of polluted environment is presented on the example of tetrade analyses Calluna vulgaris during the last hundred years in the selected regions of Slovakia. Presented results have shown significant increase of pollen abortions in relation to the years of their collections. Dynamic of impact of polluted environment on evaluated pollen tetrads for example in heavy polluted area of Žiar nad Hronom (with peak in year 1965 with 56% of abortiveness) is interesting. Surprising results with relatively high abortiveness were observed in recreation areas of the High Tatras. This test gives us opportunity to compare the impact of pollution for one hundred years retrospectively.