Analysis of the global and general principle of ecological integrity (EI) and an attempt in implementing it in the Czech Republic is the aim of the article. We need to solve several methodological problems concerning the principle of EI: briefly, can we label as core areas only zones without human impact under the present conditions of European cultural landscapes? We modified the theoretical global principle of static integrity into dynamic integrity for the purpose of the detailed counting of landscape structures. We introduced basic socio-economic typology into the Czech landscape. There are the following five landscape types: natural landscape, largely natural landscape, largely rural landscape, largely agricultural landscape, and largely urban and industrial landscape. Statistically, there is an increasing area of the first group of the most natural zones in our country. It means, according to EI, an increasing area of core zones. The source for this seems to be decreasing agriculture land area. However, there are also important socio-ecological reasons for the stability of this process.