During the years 1999-2002 mesostigmatid mites, as important part of soil mesofauna communities, were studied in two oak-hornbeam forests in the Malé Karpaty Mts. 75 species from 4 cohors (Antennophorina, Gamasina, Sejina, and Uropodina) were recorded in these sites. The mite communities in the study site Lošonec-lom quarry regularly impacted by limestone dust from the nearby quarry show greater fluctuations in species richness and abundance of mites during the year. In the study site Lošonský háj grove Nature Reserve there are evident influence of more stable microclimatic conditions in the forest. The comparison of mite communities in consecutive years through hierarchical clustering suggests, that the temperature and air humidity can have stronger influence to mesostigmatid mite assemblages than the type of soil, pH and vegetation. From the faunistical point of view, Pergamasus canestrinii was recorded for the first time in Slovakia.