Nests of birds and mammals provide ecological conditions for a rich and diverse invertebrate nidofauna. Although there are a lot of acarological and entomological studies dealing with nests, thrips have not been intensively observed yet. Therefore our research on 252 vertebrate nests according to 38 bird and 5 mammal species in Slovakia, positive in thrips, has brought original and interesting information into an untraditional field of the thysanopterology. 1,135 thrips of 38 species and Thripidae and Phlaeothripidae families were recorded in the study material. Limothrips denticornis (Haliday, 1836) (35.96%), Chirothrips manicatus (Haliday, 1836) (14.09%) and Thrips viminalis (Uzel, 1895) (9.60%) were the most dominant thrips. We also determined 2 new species for the fauna of Slovakia here: Cryptaplothrips famelicus (Priesner, 1926) and Limothrips angulicornis (Jablonowski, 1894). The highest value of an occurrence frequency (41.7%) was recorded in nests around water or even right on a water level (Acrocephalus arundinaceus, Acrocephalus palustris, Anas platyrhynchos). Because thrips dont form a typical nidicole fauna, respectively they are not nest parasites, their occurrence in the nest material probably and mainly corresponds with a passive transport on straws of various plants representing a material to build a nest. However, an active transport of mobile species can not be excluded.