The tracts of floodplain forests in southern Moravia in the alluvium of the Dyje river and Morava river were heavily impacted by anthropic activities in the last thirty years. The regulation of the two rivers, the construction of group water mains, and the specific climatic conditions markedly changed the moisture regime of soils. The process of revitalization measures was started in some parts of the floodplain forest at the beginning of the 90s. Based on both previous and very recent measurements, the paper documents the hydrological and climatic factors which affect the overall soil moisture regime in the floodplain ecosystem. They are presented results provided by the regression and correlation analysis for soil moisture, groundwater table, air and soil temperature, atmospheric precipitations.