The aim of this work was to evaluate nitrate concentrations in selected wells in the district of Levice. Sampling of water was carried out in villages: Bajka, Horná Seč, Kalná nad Hronom, Lok and Nový Tekov. The results obtained were compared with the limit values set out in Government Regulation no. 496/2010 and Decree of the Ministry of Agriculture and Ministry of Health of the Slovak Republic no. 809/9/2004. The results show that nitrate concentrations exceeded the limit value of 50 mg.l-1 in the summer period in 2011 at 30 % of samples (52.3 - 71.2 mg.l-1) in the villages of Bajka and Lok and in the autumn period only 10 % of samples (53.9 - 55.9 mg.l-1) in Bajka village. In the spring of 2012 it was up to 70 % of samples (58.1 - 97.7 mg.l-1) in the villages Bajka, Lok, Kalná nad Hronom and Nový Tekov, which is probably related to the absence of vegetation on the soil cover. In 2013, in the summer (56.7 - 85.3 mg.l-1) and autumn (53.9 - 91.5 mg.l-1), 30 % of the samples were above the limit (> 50 mg.l-1) ) in the villages Bajka and Lok. In the spring of 2014, only 20 % of samples (100.6 - 116.9 mg.l-1) were above the limit value in the village of Bajka. Low nitrate concentrations in that year were probably due to low rainfall, as a result of which nitrates were not leached into groundwater. In 2011, only 10% of samples in summer (8.8 mg.l-1) and autumn (8.2 mg.l- 1) met the nitrate limit set for infants (10 mg.l-1). Similarly, in 2013 only 10 % of samples (7.2 - 7.9 mg.l-1) taken in the municipality of Kalná nad Hronom complied with the stated limit value in summer and autumn. In 2014, up to 60 % of samples (5.2 - 8.0 mg.l-1) in the villages of Horná Seč, Kalná and Nový Tekov complied with the limit value of 10 mg.l-1 in 2014.